Haibo Jiang oral presentation (OB3-Thu3-1-3)
Correlative NanoSIMS and Electron Microscopy for Lipid Studies
1 UWA, 35 Stirling Hwy, 6009 Perth, Australia
2 UCLA, 650 Charles E. Young Dr. South, CA 90095 Los Angeles, United States
Lipids are essential molecules in organisms. They are critical components of membrane structures and also important sources for energy and carbon, and play critical roles in signaling pathways. Abnormalities in lipid metabolism can also lead to severe health conditions. However, our understanding of lipids is far less than our understanding of proteins. We are at a stage where there are tremendous potential opportunities, requiring a more concentrated effort in lipid research. There are many factors limiting the progressing of lipid studies, and one of the most important ones is the lack of powerful techniques. Current lipid research has mostly relied on conventional biochemical or biophysical techniques. While these approaches have yielded key insights, most of the time information regarding subcellular compartments and different cell types is lost.
There have been significant developments of lipid visualisation and quantification using secondary ion mass spectrometry (SIMS). The NanoSIMS, as a SIMS technique, allows high-resolution visualisation of stable isotope labelled lipids . This presentation will discuss three lipid study applications using correlative imaging with NanoSIMS and electron microscope we developed in the past two years: (A) investigating cellular cholesterol efflux mechanisms, using the 15N-ALOD4 probe for cholesterol visualisation ; (B) studying how dysferlin-deficiency  affects lipid turnover in skeletal muscles at subcellular scale; (C) examining how CD36 deficiency, using knockout mouse models, results in abnormalities in the trafficking of TRL-derived lipids.