Hua Tian oral presentation (OB3-Tue4-2-4)
Novel high energy gas cluster ion beam for mass spectrometry imaging at 1 µm lateral resolution
Chemistry Department, Pennsylvania State University, Shortlidge Road, PA 16802 University Park, United States
Biological imaging using the highest achievable lateral resolution is vital to precisely locating biomolecules at the cellular level, gaining insight into complex molecular process. However, this goal has been hindered by either limited lateral resolution or lack of chemical-specificity using other imaging techniques (e.g., MALDI and fluorescence microscopy). Secondary ion mass spectrometry (SIMS) has unprecedented lateral resolution but very limited mass range. Our work and other studies have shown that gas cluster ion beams (GCIB) produce less chemical damage yielding a larger mass range ~ m/z 3000 [1,2] than other projectiles and can achieve a focus of a few microns [3,4]. To maximize its imaging capability, a newly designed GCIB is introduced, operating at up to 80 keV and with CO2 gas, that allows submicron chemical imaging of various organic and biological systems.
Here we present the GCIB-SIMS imaging approaches for characterization of complex biological systems. On the mouse brain tissue section from the traumatic injury model, the molecular ions of cardiolipin (CL) and ganglioside up to m/z ~3000 are mapped, demonstrating the detailed distributions which has not been previously reported as shown in Figure. In various mammalian cells (e.g., HeLa), the large intact lipids (e.g., CL) are seen at the single cell level with 1 µm lateral resolution, along with intact lipids species at m/z 700~1000 and doped pharmaceutical active compounds. This workflow leads to a new level of high resolution molecular imaging, serving as a valuable tool for biological and pharmaceutical studies.
 H. Tian, L. J. Sparvero, A. A. Amoscato, A. Bloom, H. Bayır, V. E. Kagan, and N. Winograd. Analytical Chemistry, DOI: 10.1021/acs.analchem.7b00164, 2017
 H. Tian, A. Wucher and N. Winograd. Surface and Interface Analysis, 41(S1), 115-117, 2014
 Tina B. Angerer a, Paul Blenkinsopp b, John S. Fletcher. International Journal of Mass Spectrometry, 377, 591–598, 2015
 H. Tian, D. Maciążek, Z. Postawa, B. J. Garrison, N. Winograd. Journal of
The American Society for Mass Spectrometry, 27(9), 1476-1482, 2016