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SIMS21, Poland 2017 - Luke Hanley abstract

Luke Hanley oral presentation (PB2-Thu3-2-1)

VUV Postionization with fs-Laser Ablation: Implications for SNMS

Grant E. Barry, Igor V. Veryovkin, Luke Hanley

University of Illinois at Chicago - Department of Chemistry, MC 111, 845 W. Taylor St., 4500 SES, IL 60607 Chicago, United States

Laser desorption vacuum ultraviolet postionization mass spectrometry (LDPI-MS) has been demonstrated on a variety of instrumental platforms [1], but these experiments have mostly employed nanosecond pulsed lasers for desorption/ablation. Ablation with femtosecond (fs) laser pulses can remove material from a solid with minimal damage to the remaining sample, potentially allowing for both depth profiling and additionally, higher lateral resolution [2]. Furthermore, fs-laser ablation induces no more molecular fragmentation than other popular imaging ion sources operating at similar background pressures, under the proper experimental conditions [3].

Results are presented for analysis of lipids within intact slices of human pancreas tissue using both fs-LDPI-MS and fs-laser desorption ionization (fs-LDI-MS): differences in spectra with and without vacuum ultraviolet (VUV) postionization are compared and the possibility of depth profiling examined. Micron-scale occlusions within rocks and other geological samples are also mapped by fs-LDPI-MS. Other imaging modalities are employed in both cases to assist in analysis of fs-LDPI- and/or fs-LDI-MS images.

The potential for VUV postionization to enhance detection of neutral molecules by secondary neutral mass spectrometry (SNMS) will be examined in light of experimental limitations and mechanisms beyond single photon ionization of isolated molecules.

[1] A. Akhmetov, C. Bhardwaj, L. Hanley, Meth. Molec. Biol. 1203, 2015, 185.

[2] Y. Cui, I.V. Veryovkin, M.W. Majeski, D.R. Cavazos, L. Hanley, Anal. Chem. 87, 2015, 367.

[3] S. Milasinovic, Y. Cui, R.J. Gordon, L. Hanley, J. Phys. Chem. C 118, 2014, 28938.