Zdravko Siketic oral presentation (OB3-Thu3-1-6)
Lipidomic analysis of the healthy and diabetic mouse liver and serum using MeV-SIMS
Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia (Hrvatska)
One of the mass spectrometry (MS) techniques that is emerging in the recent years is MeV- SIMS – the Mass Spectrometry of Secondary Molecular Ions using MeV heavy ions for the excitation. MeV ions interact with the surface layers of the sample mostly through the electronic stopping causing desorption of intact molecular ions making determination of sample molecular composition much easier. Interaction of incident ion and sample mostly through the electronic stopping power is also a reason why secondary molecular ion yield is very high, which is a crucial prerequisite to enable molecular imaging of sensitive biological samples at the micrometer level.
In this work, the application of the MeV-SIMS imaging technique on the lipid analysis in liver and serum of healthy and diabetic mouse will be presented. For that purpose, NOD mice will be fed with high (58%) fat (HFD) or standard fat (11.4% fat) diet, until they increase body weight (to about 28 g) and achieve blood glucose level more than 7 mmol/L, measured weekly by glucometer in one blood drop taken from the tail.
Altered metabolism caused by HFD will induce fat deposition in liver vacuoles as well as hyperglycaemia (diabetes). Cryo-section of the liver tissue and serum of the same animal will be analysed by MeV-SIMS in order to analyze a possible link in a lipid composition and concentration. Moreover, mapping of fatty liver vacuoles and the tissue structure obtained by MeV-SIMS will be compared with the section of paraffin-embedded liver additionally stained with hemalaun and eosin (H&E) (a principal stains in histology) and cryo-section of the same liver stained with Sudan III (dye for lipids).